- Period: 2017
The reference materials were tested with both BCA assay and an ELISA kit to detect raw and roasted sesame seeds.
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Sesame allergy has been detected in several places around the world and has been listed in the priority allergens in Canada and Europe for example. While detection methods are being developed and optimized, reference materials are still lacking for this allergen leading to a major gap in the possibility to validate sesame detection methods in food matrices and to standardize it. The present work describes different reference materials for sesame seeds and elaborates on the advantages and the inconvenient of each developed option. The reference materials were tested with both BCA assay and an ELISA kit to detect raw and roasted sesame seeds. One of the reference materials hence developed was assessed for homogeneity and lifespan and was also used to spike sesame free food matrices.